Immunohistochemical diagnosis of urinary bladder tuberculosis
https://doi.org/10.22625/2072-6732-2014-6-1-41-46
Abstract
Diagnostics of urinary bladder tuberculosis bases on pathological verification. Standard histological staining (hematoxylin–eosin) reveals glaucomatous inflammation, but cannot estimate its etiology.
Aim of our study was to evaluate the role of complex immunohistochemical method in diagnostic of tuberculosis infection in bladder. Our study included 21 histological specimen of the resected bladder in case of nephrotuberculosis. Standard histological examination revealed specific changes in bladder tissue only in 2 cases, while immunohistochemical method with antibodies to Mycobacterium tuberculosis (MBT) demonstrated positive reaction at 5 patients. Investigation of lower urinary tract function in late postoperative period showed that patients with positive anti-MBT reaction had clinically significant chronic urinary retention, as well as their degree of urinary disorders assessed using a questionnaire IPSS-Qol was higher. Thus, the use of IHC method in combination with standard histological examination improves diagnostics of urinary bladder tuberculosis, and it may serve the predictor of long-term results of surgical treatment of microcystis.
About the Authors
S. A. Semenov
Science Research Institute of Phtisiopulmonology, Saint-Petersburg, Russia
Russian Federation
Russian Federation
N. Yu. Semenova
Science Research Institute of Phtisiopulmonology, Saint-Petersburg, Russia
Science Research Institute of Hematology and Transfusiology, Saint-Petersburg, Russia
Russian Federation
Russian Federation
K. Kh. Chibirov
Science Research Institute of Phtisiopulmonology, Saint-Petersburg, Russia
Russian Federation
Russian Federation
G. A. Raskin
Science Research Institute of Phtisiopulmonology, Saint-Petersburg, Russia
Russian Scientific Center of Radiology and Surgical Technologies, Saint-Petersburg, RussiaНесмотря
Russian Federation
Russian Federation
A. N. Muraviev
Science Research Institute of Phtisiopulmonology, Saint-Petersburg, Russia
Russian Federation
Russian Federation
References
1. Kulchavenya EV. Some aspects of Urogenital Tuberculosis. Int. J. Nephrol. Urol. 2010; 2(2):351-60.
2. Nersesyan AA, еt al. Problemy tuberkuleza. 2005; 8: 32-5.
3. Nersesyan AA, Zyuzya YuR, Merkuryeva YaA. Tuberkulez i bolezni legkikh. 2010; 7: 58-62.
4. Fligel DM, Zyuzya YuR, Alvares Figeroa MV, et al. Meditsinskiy vestnik Bashkortostana. 2010; 3: 91-2.
5. Kulchavenya YeV, Kholtobin DP, Brizhatyuk YeV. Byulleten sibirskoy meditsiny. 2011; 2: 83-7.
6. Bogin YuB, et al. Diagnostika nefrotuberkuleza v sovremennykh usloviyakh [Diagnosis of nephrotuberculosis in modern conditions] In: Vnelegochnyy tuberkulez – aktualnaya problema zdravookhraneniya [Extrapulmonary tuberculosis – current health problem]. Proceedings of Russian scientific and practical conference. St.Petersburg (Russia); 1997. р 29. Russian.
7. Shapapiro AL, Viter VI. Urologiya i nefrologiya. 1989; 1: 12-5.
8. Hemal AK, at al. Polymerase chain reaction in clinically suspected genitourinary tuberculosis: comparison with intravenous urography, bladder biopsy, and urine acid fast bacilli culture. Urology. 2000; 56(4):570-4.
9. Kulchavenya YeV, Brizhatyuk YeV. Urologiya. 2006; 3: 61-6.
10. Benchekroun A, at al. Urogenital tuberculosis. 80 . Ann Urol (Paris). 1998; 32(2): 89-94.
11. Ulrichs T, at al. Modified immunohistological staining allows detection of Zeil-Neelsen – negative Mycobacterium tuberculosis organisms and their precise ocalization in human tissue. J. Pathol. 2005; 205: 633-640.
12. Salomao R, at al. TLR signaling pathway in patients with sepsis. Shock. 2008; 30(1): 73-7.
13. Nasu, K. Pattern Recognition via the Toll-Like Receptor System in the Human Female Genital Tract / K. Nasu, H. Narahara // Human Immunology. - 2005; 66(5): 469-82.
14. Brightbill HD, at al. Host defense mechanisms triggered by microbial lipoproteins through Toll-like receptors .Science. 1999; 285(5438):732-6.
15. Means TK, Wang S, Lien E, et al. Human Toll-like receptors mediate cellular activation by Mycobacterium tuberculosis . J. Immunol. 1999; 163: 3920-7.
16. BhattK, Salgame P. Host innate immune response to Mycobacterium tuberculosis. J. Clin. Immunol. 2007; 27: 347–362.
17. Teixeira-Coelho M , at al. TLR2 deficiency by compromising p19 (IL-23) expression limits Th 17 cell responses to Mycobacterium tuberculosis. Int Immunol. 2011; 23: 89–96.
18. Fenhalls G, at al. Associations between Toll-like receptors and interleukin-4 in the lungs of patients with tuberculosis. Am. J. Respir. Cell Mol. Biol. 2003; 29: 28-38.
19. Yunkerov VI, Grigoryev SG. Mathematical and statistical analysis of medical research data. Lectures for graduate students and adjuncts. St.Petersburg (Russia): VMA; с2005. 266 р. Russian.
Review
For citations:
Semenov S.A., Semenova N.Yu., Chibirov K.Kh., Raskin G.A., Muraviev A.N. Immunohistochemical diagnosis of urinary bladder tuberculosis. Journal Infectology. 2014;6(1):41-46. (In Russ.) https://doi.org/10.22625/2072-6732-2014-6-1-41-46
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