Bocaparvovirus infection in childrenin the republic of Belarus: molecular and epidemiological aspects

Objective: To study molecular and epidemiological aspects bocaparvovirus infection at the hospitalized, children in Republic of Belarus.

Materials and methods: the studies were as part of a sentinel surveillance of influenza and. other agents of acute respiratory viral infections (ARVI) in the period. 2010 — 2018. Investigated, nasopharyngeal swabs (3907), serum. (149) hospitalized. children from 0 to 18 years with symptoms of ARVI, as well as lymphoepithelial tissue of adenoids by real-time PCR (Rotor Gene 6000, Corbett research, Australia) for the presence of respiratory DNA/RNA viruses: influenza, respiratory syncytial virus, parainfluenza virus, rhinovirus, adenovirus, metapneumovirus, bocaparvovirus, and. coronavi-ruses. The full genomic DNA sequencing of the bocaparvovirus was performed, using the commercial kit «Genome Lab DTCS Quick Start Kit» (Beckman Coulter, USA). Electrophoresis and. analysis of the reaction products were performed, on a Beckman Coulter CEQ 8000 automated, capillary DNA analyzer (Beckman Coulter, USA).

Results: the genetic material of respiratory viruses was detected, in 2781 cases (71,2%). Bocaparvovirus was detected in 337 (12,1%,) patients. Bocaparvovirus infection showed, an increase in the incidence rate in the autumn period. The most susceptible to this infection are children aged. 2 years to 4 years. The duration of viremia in these patients ranged, from 6 to 15 days. Phylogenetic analysis, the Belarusian, viruses HBoV1BLR/Mogilev/241/14, HBoV1BLR/Minsk/10/14, HBoV1BLR/Minsk/11/14, HBoV1BLR/Gomel/285/15 were combined into a separate group and showed genetic similarity with the ST2 virus. Analysis of the primary structure of the Belarusian bocaparvoviruses showed the presence of amino acid substitutions.

Conclusion: Bocaparvovirus became the fourth, virus in frequency of occurrence along with other respiratory viruses. Bocaparvovirus infection may have a severe course. All amino acid, substitutions were located, in functionally significant regions of the virus genome.

1. Allander T, Tammi MT, Eriksson M, Bjerkner A, Tiveljung-Lindell A, Andersson B. Cloning of a human parvovirus by molecular screening of respiratory tract samples. P Natl Acad Sci 2005 Jun; 102:12891 - 6.

2. Guido M, Tumolo MR, Verri T, Romano A, Serio F, De Giorgi M, De Donno A, Bagordo F, Zizza A. Human bocavirus: Current knowledge and future challenges. World J Gastroenterol 2016 Oct; 22(39):8684-8697.

3. Kapoor A, Mehta N, Esper F, et al. Identification and characterization of a new bocavirus species in gorillas. PLoS ONE 2010 July; 5(7): E11948.

4. Cheung AK, Wu G, Wang D, Bayles DO, Lager KM, Vincent AL. Identification and molecular cloning of a novel porcine parvovirus. Arch Virol. 2010 March; 155(5):801-806.

5. Arthur JL, Higgins GD, Davidson GP, Givney RC, Ratcliff RM. A novel bocavirus associated with acute gastroenteritis in Australian children. PLoS Pathog 2009 Apr; 5(4):1-11.

6. Qiu J, Soderlund — Venermo M, Young NS. Human parvoviruses. Clin Microbiol 2016 Nov; 30:43—113.

7. Kapoor A, Hornig M, Asokan A et al. Bocavirus episome in infected human tissue contains non - identical termini. PLoS ONE 2011 Jun; 6: e21362.

8. Windisch W, Pieper M, et al. Latent infection of human Bocavirus accompanied by flare of chronic cough, fatigue and episodes of viral replication in an immunocompetent adult patient. JMM 2016 Aug; 3(4): e005052.

9. Shmeleva N.P. Medicinskij zhurna. 2011.4: 129 — 131 (in Russian).

10. Sivec N.V. Associacija respiratornoj patologii s bokavi-rusom cheloveka / Sivec N.V., Shmeleva N.P., Gribkova N.V. [ Association children's respiratory pathology with human bocavirus] in Sbornik nauchnyh trudov Sovremennye problemy infekcionnoj patologii cheloveka [Modern problems of human infectious pathology]. Minsk; 2011. P 34-37. (in Russian).

11. Rowe WP, Huebner RJ, Gilmore LK, Parrott RH, Ward TG. Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture. Proc Soc Exp Biol Med 1954 Jan 84: 570-573.

12. Szalaty H, Dubowska-Inglot A. Isolation and typing of adenoviruses from adenoids. Arch Immunol Ther Exp (Warsz) 1959. 7: 615-623.