Accelerated method of identification of bacteria and micromycetes in hemocultures in children using multiplex PCR in real time
https://doi.org/10.22625/2072-6732-2019-11-4-107-112
Abstract
Objective: To evaluate the effectiveness and clinical significance of an accelerated, method, for the identification of microorganisms in blood, cultures in children using real-time multiplex PCR.
Materials and methods: A total of 72 blood, samples were obtained, from 62 children aged. 3 months to 16 years. The identification of the isolated cultures, according to the proposed, method, was carried out in real-time PCR and. also using classical culture microbiological methods.
Results: Based, on the frequency of detection and. the clinical significance of pathogens isolated from children, a panel of test systems was formed to identify them, by means of real-time PCR. It was shown that the effectiveness of the developed, method, for identification, of bacteria and. micromycetes by real-time PCR was 86,7% as compared, with the microbiological method. A significant reduction in the time of identification, of pathogens (from 48 hours to 5—7 hours) was demonstrated. In addition, the use of this method, allows, at the same time, to identify a number of antibiotic resistance genes (in particular, the genes of acquired, carbapenemases and. methicillin-resistant staphylococci).
Conclusion: The developed, method, with real-time PCR for detecting and identifying microorganisms in the blood has shown its effectiveness, clinical significance and. the possibility of using it for pediatric patients. Its use significantly shortens the time frame for obtaining results and, thus, allows timely initiation, of etiotropic therapy.
About the Authors
V. N. Chebotkevich
Russian Research Institute of Hematology and Transfusiology
Russian Federation
Competing Interests: not
Russian Federation
Saint-Petersburg
Competing Interests: not
E. A. Martens
Pediatric Research and Clinical Center for Infectious Diseases
Russian Federation
Competing Interests: not
Russian Federation
Saint-Petersburg
Competing Interests: not
S. V. Sidorenko
Pediatric Research and Clinical Center for Infectious Diseases
Russian Federation
Competing Interests: not
Russian Federation
Saint-Petersburg
Competing Interests: not
E. Е. Kiseleva
Russian Research Institute of Hematology and Transfusiology
Russian Federation
Competing Interests: not
Russian Federation
Saint-Petersburg
Competing Interests: not
S. S. Bessmeltsev
Russian Research Institute of Hematology and Transfusiology
Russian Federation
Competing Interests: not
Russian Federation
Saint-Petersburg
Competing Interests: not
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Review
For citations:
Chebotkevich V.N., Martens E.A., Sidorenko S.V., Kiseleva E.Е., Bessmeltsev S.S. Accelerated method of identification of bacteria and micromycetes in hemocultures in children using multiplex PCR in real time. Journal Infectology. 2019;11(4):107-112. (In Russ.) https://doi.org/10.22625/2072-6732-2019-11-4-107-112
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