NONPOLIO ENTEROVIRUSES WHICH CAUSED THE RISE OF ENTEROVIRUS INFECTION ON SOME TERRITORIES OF RUSSIA IN 2016

Aim: Characteristics of the peculiarities and the etiological factor of enterovirus infection on some territories of Russia in 2016.

Materials and methods: We investigated 2138 samples from the patients with enterovirus infection. The isolation and identification of enteroviruses were conducted by the virological method and by partial sequencing of the genome region VP1. Phylogenic trees were constructed according to the method of Bayesian Monte Carlo Markov Chain.

Results: Epidemic peaks of enterovirus infection were fixed on some territories of Russia. In Saratov region the morbidity index of enterovirus infection in 2016 was twice as high as the median morbidity index over previous years. The morbidity level of enterovirus meningitis – 3, 21 for 100000 of the population (77% from all the cases of enterovirus infection) was higher than on the other territories. In Kostroma region the morbidity index of enterovirus infection in 2016 was 11 times higher than the index of the previous year. On both territories the rise of morbidity depends on the active circulation of enterovirus ЕСНО30. Enteroviruses ECHO30 from Saratov region belonged to two phylogenic groups of genotype h. To one of them belonged viruses ECHO30 from Kostroma region. In Murmansk and Leningrad regions in 2016 most cases of enterovirus infection were represented by hand, foot and mouth disease (HFMD). The grouped foci of infection were registered in some preschool institutions. The etiological factor of this clinical form was Coxsackieviruses A6 belonging to different genetic variants.

Conclusion: Epidemic peaks of enterovirus infection with the prevalence of different clinical forms of the disease were provoked by different etiological factors. On territories where enterovirus meningitis prevailed strains of enterovirus ECHO30 belonging to different variants of genotype h were detected. In patients with clinical picture of HFMD from territories where this form was leading the etiological factor of infection was Coxsackievirus A6 of different genetic variants.

1. Onishchenko G.G., Novikova N.A., Efimov E.I., et al. Jurnal microbiologii. 2009; 2: 24-30 (in Russian).

2. Bichurina M.A., Pianykh V.A., Novikova N.A et al. Infectsya i immunitet. 2012; 2(4): 747-752 (in Russian).

3. Shishko L.A., Romanenkova N.I., Bichurina M.A. et al. Infectsya i immunitet. 2013; 3(2): 65-72 (in Russian).

4. Bichurina M.A., Romanenkova N.I., Golitsyna L.N. et al. Jurnal infectologii. 2014. 6(3): 84-91 (in Russian).

5. Bichurina M.A., Romanenkova N.I., Novikova N.A. et al. Jurnal microbiologii. 2014; 2: 51-58 (in Russian).

6. Ray CG. Sherris Medical Microbiology. 4th ed. NY (USA): The McGraw-Hill Companies; c2004. Chapter 12, Enteroviruses; p. 531-41.

7. Lukashev A.N., Reznik V.I., Ivanova O.E. et al. Voprosy virusologii. 2008; 53(1): 16-21 (in Russian).

8. Romanenkova N.I., Bichurina M.A., Rozaeva N.R. Zhurnal mikrobiologii. 2011; 6: 32-36 (in Russian).

9. Khetsuriani N, Lamonte-Fowlkes A, Oberste S, et al. Enterovirus surveillance – United States, 1970 – 2005. Morbid. Mortal. Wkly Rep. 2006; 55 (8):1–20.

10. Zang Y, Wang D, Yan D, et al. Molecular evidence of persistent epidemic and evolution of subgenotype B1coxsaсkievirus A16-associated hand, foot, and mouth disease in China. J. Clin. Microbiol. 2010 Feb; 48 (2): 619-22.

11. Osterback R, Vuorinen T, Linna M, et al. Coxsackievirus A6 and Hand, Foot, and Mouth Disease, Finland. Emerg Infect Dis. 2009 Sep; 15(9):1485-88.

12. Bracho MA, Gonza.lez-Candelas F, Valero A, et al. Enterovirus co-infections and onychomadesis after hand, foot, and mouth disease, Spain, 2008. Emerg. Infect. Dis. 2011 Dec; 17 (12): 2223-31.

13. He YQ, Chen L, Xu WB, et al. Emergence, Circulation, and Spatiotemporal Phylogenetic Analysis of Coxsackievirus A6- and Coxsackievirus A10-Associated Hand, Foot, and Mouth Disease Infections from 2008 to 2012 in Shenzhen, China. J. Clin. Microbiol. 2013 Nov; 51 (11): 3560-66.

14. Bian L, Wang Y, Mao Q, et al. Coxsackitvirus A6: a new emerging pathogen causing hand, foot and mouth disease outbreaks worldwide. Expert Rev. Anti Infect. Ther. 2015 Jun; 13 (9): 1061-71.

15. Polio laboratory manual. WHO/IVB/04.10. Geneva (Switzerland): World Health Organization; c 2004. 157 p.

16. Nix WA, Oberste MS, Pallansch MA. Sensitive seminested PCR amplification of VP1 sequences for direct identification of all enterovirus serotypes from original clinical specimens. Clin. Microbiol. 2006 Aug; 44 (8): 2698-704.

17. Tamura K, Peterson D, Peterson N, et al. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol. Biol. Evol. 2011 Oct; 28 (10): 2731-39.

18. Drummond AJ, Suchard MA, Xie D, Rambaut A (2012) Bayesian phylogenetics with BEAUti and the BEAST 1.7. Mol Biol Evol. 2012 Feb; 29 (8): 1969-73.

19. Novikova N.A., Golitsyna L.N., Fomina S.G., Efimov E.I. Zhurnal mikrobiologii. 2013; 1: 75–78 (in Russian).

20. Golitsyna L.N., Zverev V.V., Epifanova N.V. et al. Zabolevaemost, etiologicheskaya structura i voprosy proilaktiki enterovirusnoy (nepolio) infektsii. Information bulletin (Internet). Nizhny Novgorod; 2017. [cited 2017 Jun 6]; 4: 25-31. Available from: http://www.nniiem.ru/file/news/2017/byulleten-evi-n42017-sayt.pdf (in Russian).

21. Bailly JL, Mirand A, Henquell C, et al. Phylogeography of circulating populatios of human echovirus 30 over 50years: nucleotide polymorphism and signature of purifying selection in the VP1 capsid protein gene. Infect. Genet. Evol. 2009 Jul; 9 (4): 699-708.

22. Golitsyna L.N., Novikova N.A., Fomina S.G., et al. Sbornik trudov VIII Vserossiyskoy nauchno-prakticheskoy konferentsii “Molekulyarnaya diagnostika” [The 8th Russia-wide scientific conference with international participation “Molecular Diagnostics 2014”]. Vol.1. Moscow; 2014. P. 416-17 (in Russian).

23. Yang XH, Yan YS, Weng YW Molecular epidemiology of Echovirus 30 in Fujian, China between 2001 and 2011. J. Med. Virol. 2013 Apr; 85 (4): 696-702.

24. Sapega E.Y., Trotsenko O.E., Butakova L.V. Zabolevaemost, etiologicheskaya structura i voprosy proilaktiki enterovirusnoy (nepolio) infektsii. Information bulletin (Internet). Nizhny Novgorod; 2017. [cited 2017 Jun 6]; 4: 25-31. Available from: http://www.nniiem.ru/file/news/2017/byulleten-evi-n42017-sayt.pdf (in Russian).

25. Golitsyna L.N., Zverev V.V., Parfenova O.V., et al. Dalnevostochnyi zhurnal infektsionnoi patologii. 2015; 28: 12-20 (in Russian).

26. Puenpa J, Vonqpunsawad S, Osterback R, et al. Molecular epidemiology and evolution of human coxsackievirus A6. J. Gen. Virol. 2016 Dec; 97 (12): 3225-31.

27. Novik E.S., Reznik V.I., Karavyanskaya T.N. et al. Dalnevostochnyi zhurnal infektsionnoi patologii. 2009; 14: 6-13 (in Russian).

28. Hovi T, Stenvik M, Partanen H, Kangas A. Poliovirus surveillance by examining sewage specimens. Quantitative recovery of virus after introduction into sewage at remote upstream location. J. Epidemiol. Infect. 2001 Aug; 127 (1): 101–6.