serologic markers of Epstein-Barr virus reactivation in the conditions of viral encephalitis in young patients
https://doi.org/10.22625/2072-6732-2015-7-1-70-74
Abstract
The reactivation of Epstein-Barr virus on the background of some basic disease revealed during the development of encephalitis of various etiology could act as a co-factor to trigger the process of demyelinazation of central nervous system tissues.
Purpose: to clarify the role of persistent Epstein-Barr virus infection in children with the diagnosis of viral encephalitis. Materials and methods: there was performed the study of biological material (blood serum) of 29 children aged from 1 to 14 years old with the diagnosis of viral encephalitis during the period of 2012–2013. The algorithm of the study included serological methods on the basis of the enzymelinked immunosorbent assay manufactured by Joint-Stock Company «Vector-Best» (Novosibirsk) on the open-type apparatus «Lasurit» of «Dynex Technologies Inc.» company (USA) and blot-technologies with the use of Western-blotting manufactured by Euroimmun AG, Germany on the apparatus «AUTOBLOTT 3000» of «MedTec, Inc.» company (USA).
Results: It was determined that during the testing of the late phase Epstein-Barr antibodies (IgG-NA and IgG-VCA) with the indicators of high avidity index, the blot-technology use also revealed IgG class antibodies to the early phase antigenes of this virus (EA-R, and EA-D), synthesized within the period of not longer than three weeks beginning from the disease onset. This allowed to assume the development of an active phase of chronic Epstein-Barr virus infection in the observed patients. The antibodies to an early antigen were noticed particularly often in the case of encephalitis caused by varicella zoster virus that composed 62,5% of total number of cases with the development of encephalitis of given etiology.
Conclusion: in the case of associated infections by those viruses among which there are etiologically significant ones for encephalitis development, Epstein-Barr virus, the infection by which occurred earlier, can be activated and influence the development of the pathologies connected with demyelinazation of central nervous system tissue.
About the Authors
O. V. Goleva
Scientific Research Institute of Children’s Infections of FMBA of Russia, Saint-Petersburg, Russia
Russian Federation
Russian Federation
E. A. Murina
Scientific Research Institute of Children’s Infections of FMBA of Russia, Saint-Petersburg, Russia
Russian Federation
Russian Federation
Z. A. Osipova
Scientific Research Institute of Children’s Infections of FMBA of Russia, Saint-Petersburg, Russia
Russian Federation
Russian Federation
References
1. Котлова, В.Б. Перинатальная Эпштейна – Барр вирусная инфекция / В.Б. Котлова // Вестник новых медицинских технологий. – 2013. – Т. 20, No 2. – С. 95–98.
2. Филатова, Е.Н. Современные подходы к моделированию герпесвирусной инфекции / Е.Н. Филатова, О.В. Уткин // Журнал МедиАль. – 2014. – No 2 (12). – С. 172–197.
3. Исаков, В.А. Герпес: патогенез и лабораторная диагностика : руководство для врачей / В.А. Исаков, В.В. Борисова, Д.В. Исаков – СПб.: Изд-во «Лань», 1999. – 192 с.
4. Орлова, С.Н. Современные аспекты диагностики острой вирусной инфекции Эпштейна – Барр / С.Н. Орлова [и др.] // Вестник Ивановской медицинской академии. – 2009. – Т.14, No 4. – С. 40–44.
5. Долгих, Т.И. Особенности течения герпесвирусных инфекций у детей / Т.И. Долгих, Е.Ю. Минакова // Детские инфекции. – 2012. – Т. 11, No 3. – С. 56–58.
6. Adenot, M. Clinical presentation of severe viral encephalitis with known causative agents in children / М. Adenot [et al.] // J. Child Neurol. – 2014. – V. 29. – P. 1508–1518.
7. Fujimoto H. Epshtein-Barr virus infections of the central nervous system / H. Fujimoto [et al.] // Intern. Med. – 2003. – V. 42 (1). – Р. 33–40.
8. Спирин, Н.Н. Диагностика, дифференциальная диагностика и принципы терапии острого рассеянного энцефаломиелита / Н.Н. Спирин [и др.] // Неврология и ревматология. Приложение к журналу Consilium Medicum. – 2008. – No 2. – С. 23–28.
9. Обрядина, А.П. Использование метода определения авидности IgG в лабораторной диагностике инфекционных заболеваний / А.П. Обряднина Е.О. Копнина // Дальневосточный журнал инфекционной патологии. – 2006. – No 9. – С. 98–99.
Review
For citations:
Goleva O.V., Murina E.A., Osipova Z.A. serologic markers of Epstein-Barr virus reactivation in the conditions of viral encephalitis in young patients. Journal Infectology. 2015;7(1):70-74. (In Russ.) https://doi.org/10.22625/2072-6732-2015-7-1-70-74
Views:
2150
.png)
.png)
Email this article 