The results of virological surveillance for intrauterine infections in Saint-Petersburg

The purpose of researchis the determiningthe etiological structure ofintrauterine infections in Saint-Petersburg pediatric patients, pregnant women andinfants born to them using a variety of virological methods.

Methods: serum from 164 children aged from 1 month to 14 years with diagnosis of «intrauterine infection». Serum from 80 pregnant women, collected in each trimester (total – 240 samples, their 42 children (at the age of 1–2 and 4–6  months of life, total – 82 samples). Immunoglobulin Mand G (IgM and IgG)to herpes virus type 1, cytomegalovirus (CMV), Toxoplasma gondii, mycoplasma and chlamydia, rubella, Epstein-Barr virus (EBV) and parvovirus B19, as well as IgG avidity,were determined by ELISA in all these samples. Theimmunoblot (Western blot), using the «Immunoblot2000» with test kits from «Euroimmun AG» (Germany), was applied to confirm cases. Statistical analysis wasperformed with theprograms Microsoft Excel, Statistica6.

Results: cytomegalovirus, herpes virus 1st type and Epstein-Barr virus infections are dominate in the structure of intrauterine ones (45%, 23% and 14%, respectively). Laboratory evidence ofreactivationof cytomegalovirus (35% ofpregnant womenin the 2nd and/or 3rd trimesters) and acuteparvovirus infection (15% of cases) were found. Specific IgM to cytomegalovirus were detected in 6,2% ofchildren in the firstsix months of life.

Conclusions: with the aim of early detection of cytomegalovirus reactivation and acute parvovirus infection it isnecessary to monitor pregnant women with the definition of specific IgM, IgG and avidity IgG. The procedure to using immunoblotting in the diagnosis of intrauterine infections
needs to be further study.

1. Grinow A., Osborn J., Sazerlend Sh., editors. Congenital, perinatal and neonatal infections. M.; c2007 (in Russian). 288 p.

2. Nisevich L.L., Talalayev A.G., Kask L.N., Mironyuk O.V. et al. Voprosy sovremennoy pediatrii. 2002; 1( 4): 9–13.

3. Vasilyev V.V., MurinaYe.A., Sidorenko S.V., Mukomolova A.L. et al.Zhurnalinfektologii. 2011; 3( 4): 26-33.

4. LobzinYu.V., Vasilyev V.V., Skripchenko N.V., Ivanova V.V., Babachenko I.V., Tekhova I.G.et al. Zhurnal infektologii. 2010; 2(2): 14-24.

5. Zaplatnikov A.L., Korneva M. Yu., Korovina N. A.et al.Russkiymeditsinskiyzhurnal. 2005; 13 (1): 45-7.

6. Korovina N. A., Zaplatnikov A. L., Cheburkin A. V., Zakharova I. N. Cytomegalovirus infection in young children (clinical picture, diagnosis, modern therapy options): Manual for doctors.М., c1999.

7. Lobzin Yu. V., Vasilyev V. V. Rossiyskiy meditsinskiy zhurnal. 2001; 5: 40-1.

8. VasilyevV.V., MurinaYe.A., KvetnayaA.S. etal. Rossiyskiy semeynyy vrach. 2013;17( 1): 36

9. Lanari M. , Papa I., Venturi V. , Lazzarotto T. et al. Congenital infection with human herpesvirus 6 variant B associated with neonatal seizures and poor neurological outcome. J Med Virol. 2003; Aug; 70(4): 628-632.

10. Hall C. B., Caserta M. T., Schnabel K. C., Boettrich C. et al. Congenital infections with human herpesvirus 6 (HHV6) and human herpesvirus 7 (HHV7). J Pediatr. 2004 Oct; 145(4): 472-7.

11. Problems of intrauterine infection in fetus and newborn. M; с2000. The results of early and prolonged catamnesis in children born from mothers with herpes infection; р. 273-5.

12. Zaplatnikov A.L. Kliniko-patogeneticheskoye obosnovaniye immunoterapii i immunoprofilaktiki infektsionnovospalitelnykh zabolevaniy u detey [Clinical and pathogenetic substantiation of immunotherapy and immunoprophylaxis of infectious and inflammatory diseases in children]: [master›s thesis]. Moscow (Russia); 2003.

13. Korneva M. Yu., Korovina N. A., Zaplatnikov A.L.et al. Rosciyskiy vestnik perinatologi i ipediatrii. 2005; 2: 48-52.

14. Nisevich L. L. Detskiy doktor. 2000;5: 26-30.

15. Volodin N. N., editor. Protocols of diagnosis, treatment and prevention of intrauterine infections in newborns М., c2007 (in Russian). 288 p

16. Fowler K. W., Stagno S., Pass R. F. et al. The outcome of congenital cytomegalic virus infection in relation to maternal antibody status. N Engl J Med; 1992; 326: 663-7.

17. Remington J. S., Klein J. O., editors. Infectious Disease of the Fetus and Newborn Infant. 5thed. Philadelphia, PA: WBSaundersCo; c2001. 389-424 p

18. Tsaregorodtsev A. D., Ryumina I. I. Rosciyskiy vestnik perinatologii i pediatrii. 2001; 46 (2): 4-7.

19. Shabalov N. P. Pediatriya. 2000; 1: 87-91.