Markers infectious agent in the cord blood samples public register of donors

Objective. To evaluate the distribution of markers of infectious agents in umbilical cord blood samples Pokrovskij public stem cell bank donor registry for five years (2009 – 2013).

Materials and Methods. 3533 plasma samples were investigated
after selection during cord blood processing procedure for allogeneic use in Pokrovskij stem cell bank. All plasma samples were investigated in accordance with the Order of the Ministry of Health № 325 – 2003 by enzymelinked immunoassay method. In addition, during the period from November 2011 to December 2013 1030 plasma samples of umbilical cord blood were examined for the presence of HCV RNA, the RNA of HIV and HBV DNA.

Results. Markers of the agents above have not been found in the plasma of 481 samples (13.6%). During the described period, no significant change in the share of samples containing antibodies to cytomegalovirus and toxoplasmosis (cytomegalovirus – 1978 samples (56%), Toxoplasma gondii – 112 samples (3.2%), 825 samples (23.4%) cytomegalovirus and Toxoplasma gondii simultaneously) were registered. 137 samples (3.9%) were subjected to utilization in connection with detection of antibodies to HbcorAg – 116 samples (3.3%), antibodies to HCV – five samples (0.14%), and antibodies to Treponema pallidum – 16 samples (0.45%).

Conclusion. The introduction of an additional method of polymerase chain reaction for the detection of nucleic acids of hepatitis viruses B, C, human immunodeficiency virus, along with study of cord blood samples by enzyme-linked immunoassay improve the quality of the control of the transmission of blood-borne infections.

1. Tjumina O.V. Umbiblical cord blood: processing, storage, transplantation and regenerative medicine / Tjumina O.V., Hurcilava O.G., Smoljaninov A.B. – SPb.: Science, 2012 (in Russian).

2. About the development of cells technologies in Russian Federation: Order of Ministry of Health RF 25 June 2003. № 325 // Ros. gaz. -2003. – 12 aug. (in Russian).

3. About blood and its components donation: The Law of Russian Federation 09.06.1993 № 5142-1 // Vedomosti SND i VS RF. 1993. – № 28. – St. 1064 (in Russian).

4. NetCord-FACT International Cord Blood Standards for Cord Blood Collection, Banking, and Release for Administration. – Fourth Edition. – January, 2010. – 87 p. (in English).

5. Kodenev A.T., Gubanova M.N., Zhiburt E.B Vestnik sluzhby krovi Rossii. 2010; 2:13-6 (in Russian).

6. Tabor E, Epstein JC. NAT screening of blood and plasma donations: evolution of technology and regulatory policy. Transfusion 2002 Sep;42(9): 1230-6 (in English).

7. Ob utverzhdenii pravil i metodov issledovanij i pravil otbora obrazcov donorskoj krovi, neobhodimyh dlja primenenija i ispolnenija tehnicheskogo reglamenta o trebovanijah bezopasnosti krovi, ee produktov, krovezameshhajushhih rastvorov i tehnicheskih sredstv, ispol'zuemyh v transfuzionno-infuzionnoj terapii: Postanovlenie Pravitel'stva RF 31 deс. 2010 № 1230 // Sobr. zakonodatel'stva RF. – 2011. – №3. – S. 553. (in Russian).

8. Beljakova V.V., Gukasjan I.A., Momotjuk K.S., Majorova O.A., Kuznecov O.E. Vestnik sluzhby krovi Rossii. 2012; 1: 9 – 12 (in Russian).

9. HBV NAT and anti-NVs-testing increases blood safety / Kurt Ros V, Veber M, Petersen D, Drosten K, Bur S, Sirajs V, Vajhert V, Hedzhes D, Zajfrid J// NAT-minipul-geneskrining of blood based on international standarts of WHO- guarantee of viral and bacterial safety of recipients. Methodological collected book edited by N.A. Fedorov – M. – Novosibirsk – Frankfurt-na-Majne, 2004 (in Russian).

10. Stramer SL, Wend U, Candotti D, Foster GA, Hollinger FB, Dodd RY, Allain JP, Gerlich W. Nucleic Acid Testing to Detect HBV Infection in Blood Donors. NEJM. 2011 Jan; 364(3): 236-47. (in English).

11. Ammosov A.D. Hepatitis В. : Novosibirsk; 2006 (in Russian).

12. Gerlich WH, Wienzek S, Wend UC, Dickmeiss E, Saniewski M, Chudy M, Glebe D, Lattermann A, Schittler CG, Harritshoj L, Ullum H, Willems WR. Hepatitis В virus variants HbSAg negative, anti-Hbc positive blood donors: infectivity, pathogenicity and variability. Vox Sang. 2010 Jul; 99(Suppl.1):22. (in English).