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Antigen induced production of υ-interferon ex vivo, in the peripheral blood of patients with active pulmonary tuberculosis

https://doi.org/10.22625/2072-6732-2013-5-2-22-31

Abstract

Tuberculosis (TB) is one of the most significant problems in the Russian Health Care. Russia remains on the list of the 22 countries with a high TB incidence and on the third place in the world with a high prevalence of Drug Resistant TB [1]. It is urgently needed to develop new TB diagnostic methods as well as effective measures of the specific TB prevention, including a development of the novel vaccines, so we have to know better about the most immunogenic antigens of Mycobacterium Tuberculosis. We studied the Interferon-Q production in the whole blood after stimulating immune response with different proteins of Mycobacterium Tuberculosis in patients with active TB. The study results permitted us to evaluate the immunogenicity of the previously known proteins (Ag85a и ESAT-6) in comparison to the recently identified ones (Rv2957, Rv2958c и Rv0447), analyzing simultaneously their relation to tuberculin, as well as to antigens of the different viruses (Human Immunodeficiency Virus, Cytomegalovirus, Epstein-Barr Virus, Influenza Virus). Protein Rv2958c, unlike protein ESAT-6, showed the high immunogenicity in comparison to tuberculin. The expressed immunogenicity of protein Rv2958c might be indicated a possible greatest specificity of immune response to this antigen in TB patients. Meanwhile, bacillary tuberculosis was strongly associated with low immune response to this protein. Also we were found statistical differences in immune responses of patients to the different Mycobacterium Tuberculosis antigens depending on the drug sensitivity. In addition it was interesting to know about a significantly low immune response of patients with Drug Resistant TB to protein pp65 CMV.

About the Authors

Z. M. Zagdyn
Saint-Petersburg Science Research Institute of Phthisiopulmonology, Saint-Petersburg
Russian Federation


A. A. Lutskiy
Science Research Institute of Children’s Infections, Saint-Petersburg
Russian Federation


A. A. Zhirkov
Science Research Institute of Children’s Infections, Saint-Petersburg
Russian Federation


O. A. Makarenko
Science Research Institute of Children’s Infections, Saint-Petersburg
Russian Federation


D. Ferrara
Karolinska University Hospital, Stockholm, Sweden Department of Microbiology, Tumor and Cell Biology, Karolinska University, Stockholm, Sweden University of Perugia, Perugia, Italy Division Therapeutic Immunology, Karolinska University, Stockholm, Sweden
Russian Federation


R. Axelsson-Robertson
Karolinska University Hospital, Stockholm, Sweden
Russian Federation


I. Magalhaes
Department of Microbiology, Tumor and Cell Biology, Karolinska University, Stockholm, Sweden
Russian Federation


N. A. Skrynnik
Saint-Petersburg Regional Antituberculosis Dispensary №12, Saint-Petersburg
Russian Federation


V. N. Shabalin
Zeleniy Holm Tuberculosis hospital, Zeleniy holm, Leningrad Region
Russian Federation


H. N. Dzhumaeva
Zeleniy Holm Tuberculosis hospital, Zeleniy holm, Leningrad Region
Russian Federation


M. Maeurer
Karolinska University Hospital, Stockholm, Sweden Department of Microbiology, Tumor and Cell Biology, Karolinska University, Stockholm, Sweden Division Therapeutic Immunology, Karolinska University, Stockholm, Sweden
Russian Federation


S. V. Sidorenko
Science Research Institute of Children’s Infections, Saint-Petersburg
Russian Federation


Yu. V. Lobzin
Science Research Institute of Children’s Infections, Saint-Petersburg
Russian Federation


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Review

For citations:

Zagdyn Z.M., Lutskiy A.A., Zhirkov A.A., Makarenko O.A., Ferrara D., Axelsson-Robertson R., Magalhaes I., Skrynnik N.A., Shabalin V.N., Dzhumaeva H.N., Maeurer M., Sidorenko S.V., Lobzin Yu.V. Antigen induced production of υ-interferon ex vivo, in the peripheral blood of patients with active pulmonary tuberculosis. Journal Infectology. 2013;5(2):22-31. (In Russ.) https://doi.org/10.22625/2072-6732-2013-5-2-22-31

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