Characteristics of intestinal microbiota of infants according to data of sequencing of the 16s RRNA gene
https://doi.org/10.22625/2072-6732-2017-9-2-23-28
Abstract
The article presents the results of a single study the intestinal microbiota of healthy infants. Study was performed 11 fecal samples, followed by targeted sequencing the amplified sites on the 16S rRNA gene Miseq platform (Illumina, USA), according to standard protocols. Total 600 unique OTU (operation taxonomic units), grouped into 7 bacterial phyla (Phylum) have been identified. Each sample had an average of 190±80 OTU at the species level. Established predominance of gram-positive anaerobic bacteria (71±23%). Quantitative phylum distribution was as follows: phylum Firmicutes – 43±15% (represented by Clostridium spp., Blautia spp., Lactobacillus spp., Enterococcus spp. and Veillonella spp.), The phylum Actinobacteria – 38±10% (of which more than 90% the OTU were represented Bifidobacterium spp.) and the phylum Proteobacteria – 15±8% (represented by the family Enterobacteriaceae). Representatives of phylum Bacteroidetes been identified (7–15%) in only three of eleven samples. All samples characterized by low species diversity, Shannon index and criterion α-diversity were in the range of 1,5–4,2, and 3–20, respectively. Children who are breastfed, observed a higher number of representatives of Proteobacteria, compared with children on artificial feeding. Noted the influence of adverse factors of pregnancy mothers (antibiotics, respiratory infection) on the composition of the intestinal microbiota of children, which reflected in the dominance of Klebsiella pneumoniae in one case, and the dominance of Enterococcus durans otherwise.
About the Authors
N. V. Gonchar
Pediatric Research and Clinical Center for Infectious Diseases;
North-Western State Medical University named after I.I. Mechnikov
Russian Federation
Saint-Petersburg
Russian Federation
Saint-Petersburg
I. V. Babachenko
Pediatric Research and Clinical Center for Infectious Diseases;
Saint-Petersburg State Pediatric Medical University
Russian Federation
Saint-Petersburg
Russian Federation
Saint-Petersburg
V. V. Gostev
Pediatric Research and Clinical Center for Infectious Diseases
Russian Federation
Saint-Petersburg
Russian Federation
Saint-Petersburg
O. M. Ibragimova
Pediatric Research and Clinical Center for Infectious Diseases
Russian Federation
Saint-Petersburg
Russian Federation
Saint-Petersburg
References
1. Turroni F, Foroni E, Pizzetti P, Giubellini V, Ribbera A, Merusi P, Cagnasso P, Bizzarri B, de›Angelis GL, Shanahan F et al: Exploring the diversity of the bifidobacterial population in the human intestinal tract. Appl Environ Microbiol 2009, 75(6):1534-1545.
2. Structure, function and diversity of the healthy human microbiome. Nature 2012, 486(7402):207-214.
3. Arrieta MC, Stiemsma LT, Amenyogbe N, Brown EM, Finlay B: The intestinal microbiome in early life: health and disease. Front Immunol 2014, 5:427.
4. Gritz EC, Bhandari V: The human neonatal gut microbiome: a brief review. Front Pediatr 2015, 3:17.
5. Matamoros S, Gras-Leguen C, Le Vacon F, Potel G, de La Cochetiere MF: Development of intestinal microbiota in infants and its impact on health. Trends Microbiol 2013, 21(4):167-173.
6. Koenig JE, Spor A, Scalfone N, Fricker AD, Stombaugh J, Knight R, Angenent LT, Ley RE: Succession of microbial consortia in the developing infant gut microbiome. Proc Natl Acad Sci U S A 2011, 108 Suppl 1:4578-4585.
7. Fallani M, Amarri S, Uusijarvi A, Adam R, Khanna S, Aguilera M, Gil A, Vieites JM, Norin E, Young D et al: Determinants of the human infant intestinal microbiota after the introduction of first complementary foods in infant samples from five European centres. Microbiology 2011, 157(Pt 5):1385-1392.
8. Lozupone CA, Stombaugh J, Gonzalez A, Ackermann G, Wendel D, Vazquez-Baeza Y, Jansson JK, Gordon JI, Knight R: Meta-analyses of studies of the human microbiota. Genome Res 2013, 23(10):1704-1714.
9. Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Pena AG, Goodrich JK, Gordon JI et al: QIIME allows analysis of high-throughput community sequencing data. Nat Methods 2010, 7(5):335-336.
10. Keegan KP, Glass EM, Meyer F: MG-RAST, a Metagenomics Service for Analysis of Microbial Community Structure and Function. Methods Mol Biol 2016, 1399:207-233.
11. Million M, Lagier JC, Yahav D, Paul M: Gut bacterial microbiota and obesity. Clin Microbiol Infect 2013, 19(4):305-313.
12. Jain N, Walker WA: Diet and host-microbial crosstalk in postnatal intestinal immune homeostasis. Nat Rev Gastroenterol Hepatol 2015, 12(1):14-25.
13. Power SE, O’Toole PW, Stanton C, Ross RP, Fitzgerald GF: Intestinal microbiota, diet and health. Br J Nutr 2014, 111(3):387-402.
14. Gomez-Llorente C, Plaza-Diaz J, Aguilera M, MunozQuezada S, Bermudez-Brito M, Peso-Echarri P, Martinez-Silla R, Vasallo-Morillas MI, Campana-Martin L, Vives-Pinera I et al: Three main factors define changes in fecal microbiota associated with feeding modality in infants. J Pediatr Gastroenterol Nutr 2013, 57(4):461-466.
15. Aagaard K, Ma J, Antony KM, Ganu R, Petrosino J, Versalovic J: The placenta harbors a unique microbiome. Sci Transl Med 2014, 6(237):237ra265.
16. Ardissone AN, de la Cruz DM, Davis-Richardson AG, Rechcigl KT, Li N, Drew JC, Murgas-Torrazza R, Sharma R, Hudak ML, Triplett EW et al: Meconium microbiome analysis identifies bacteria correlated with premature birth. PLoS One 2014, 9(3):e90784.
17. Yatsunenko T, Rey FE, Manary MJ, Trehan I, Dominguez-Bello MG, Contreras M, Magris M, Hidalgo G, Baldassano RN, Anokhin AP et al: Human gut microbiome viewed across age and geography. Nature 2012, 486(7402):222-227.
Review
For citations:
Gonchar N.V., Babachenko I.V., Gostev V.V., Ibragimova O.M. Characteristics of intestinal microbiota of infants according to data of sequencing of the 16s RRNA gene. Journal Infectology. 2017;9(2):23-28. (In Russ.) https://doi.org/10.22625/2072-6732-2017-9-2-23-28
Views:
1497
.png)
.png)
Email this article 