Forecasting Model of Gene Enzyme Polymorphism Detoxification in Patients Suffered from HFRS
Abstract
Aim: to study gene enzyme polymorphism of xenobiotic detoxification in patients suffered from HFRS influenced by disease severity
Proceedings : Molecular genetic checkup has been done in 292 patients suffered from HFRS and 426 seronegative donors.DNA samples isolated from lymphocytes of peripheral gene enzyme were used for molecular genetic checkup. Phenic-chloroform extraction method was applied to isolate DNA. The given DNA was used for polymerase chain reaction of DNA synthesis. Polymorphous CYP1A1 and GSTP1 gene locus analysis was performed on an automatic basis by polymerase chain reaction of DNA synthesis in a thermal cycle «Terzik» produced «DNK–techologiya» ( Moscow city) with the use of locus specific and oligonucleotide primers.
Outcomings: Glutathion-S-transferase class π with A313G locus of AG heterozygous genotype is typical for people of Bashkortostan due to underlying risk for HFRS. A combination of genotypes in the form of cytochrome P-450A1 with polymorphous locus A2455G and glutathione-S-transferase class π with A313G locus of AG can be found only in case of severe form of HFRS.
About the Authors
G. M. Hasanova
Bashkir State Medical University, Ufa, Russia
Bashkir State University, Ufa, Russia
Russian Federation
Russian Federation
A. V. Tutel’jan
Central Science Research Institute of Epidemiology, Moscow, Russia
Russian Federation
Russian Federation
D. A. Valishin
Bashkir State Medical University, Ufa, Russia
Russian Federation
Russian Federation
A. N. Hasanova
Bashkir State Medical University, Ufa, Russia
Russian Federation
Russian Federation
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Review
For citations:
Hasanova G.M., Tutel’jan A.V., Valishin D.A., Hasanova A.N. Forecasting Model of Gene Enzyme Polymorphism Detoxification in Patients Suffered from HFRS. Journal Infectology. 2016;8(1):73-78. (In Russ.)
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