jofinЖурнал инфектологииJournal Infectology2072-6732IPO “АIDSSPbR"10.22625/2072-6732-2017-9-3-81-91jofin-637Research ArticleВирусологияVirologyФРАГМЕНТЫ, ГОМОЛОГИЧНЫЕ БЕЛКАМ СИСТЕМЫ ГЕМОСТАЗА ЧЕЛОВЕКА, В БЕЛКАХ ВИРУСОВ, ВЫЗЫВАЮЩИХ ОРЗ ИЛИ ЗАБОЛЕВАНИЯ, СХОДНЫЕ С НИМИ ПО КЛИНИЧЕСКОЙ СИМПТОМАТИКЕHOMOLOGY BETWEEN SEGMENTS OF HUMAN HEMOSTATIC PROTEINS AND PROTEINS OF VIRUSES WHICH CAUSE ACUTE RESPIRATORY INFECTIONS OR DISEASES WITH SIMILAR SYMPTOMSЖилинскаяИ. Н.ZhilinskayaI. N.

Санкт-Петербург 

Жилинская Ирина Николаевна – ведущий научный сотрудник, доктор биологических наук, старший научный сотрудник

irina@influenza.spb.ruПрочухановаА. Р.ProchukhanovaA. R.

Санкт-Петербург 

Прочуханова Анна Ростиславовна – старший научный сотрудник, кандидат биологических наук

irina@influenza.spb.ruФадеевА. В.FadeevA. V.

Санкт-Петербург 

Фадеев Артем Викторович – научный сотрудник 

artem.fadeev@gmail.ruХарченкоЕ. П.KharchenkoE. P.

Санкт-Петербург 

Харченко Евгений Петрович – ведущий научный сотрудник, доктор биологических наук

neuro.children@mail.ruНаучно-исследовательский институт гриппаРоссияScience Research Institute of UnfluenzaRussian FederationИнститут эволюционной физиологии и биохимии им. И.М. СеченоваРоссияInstitute of Evolutionary Physiology and Biochemistry named after I.M. SechenovRussian Federation201713102017938191Copyright © Жилинская И.Н., Прочуханова А.Р., Фадеев А.В., Харченко Е.П., 20172017Жилинская И.Н., Прочуханова А.Р., Фадеев А.В., Харченко Е.П.Zhilinskaya I.N., Prochukhanova A.R., Fadeev A.V., Kharchenko E.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.niidi.ru/jofin/article/view/637Цель

Цель: выявить в структуре белков исследуемых вирусов фрагменты, гомологичные фрагментам белков системы гемостаза человека, и оценить возможную роль белков системы гемостаза человека в репродукции исследуемых вирусов.

Материалы и методы

Материалы и методы: для сравнительного компьютерного анализа были использованы вирусы гриппа В (В/Астрахань/2/2017 ), коронавирусы (HCov229E и SARS-Co), аденовирусы серотипа 1 (adenoid 71), кори (ICHINOSE-BA) и краснухи (Therien). Источником первичных структур белков анализируемых вирусов и 41 белка системы гемостаза человека служили общедоступные в Интернете базы данных www.ncbi.nlm.nih.gov и www.nextprot.org соответственно. Поиск гомологичных последовательностей в структуре вирусных белков и белков гемостаза осуществляли путем сравнения в них фрагментов длиною в 12 аминокислот, принимая родственными те из них, которые проявляли идентичность по ≥ 8 позициям.

Результаты

Результаты: показано, что в структуре исследуемых вирусных белков обнаружены фрагменты, мимикрирующие ряд белков системы гемостаза, при этом значительная часть этих фрагментов гомологична факторам коагуляции крови (за исключением белков аденовируса). Следует отметить, что усиление вирулентности вируса (как в случае коронавируса SARS-Co) коррелирует с увеличением количества фрагментов, гомологичных белкам гемостаза.

Заключение

Заключение: система гемостаза играет важную роль в репродукции исследуемых вирусов, что является новым аспектом их патогенеза и подтверждается данными литературы о взаимосвязи системы гемостаза с развитием воспалительных и иммунных реакций при вирусных инфекциях.

Objectives

Objectives: To identify homologous segments of human hemostatic and viral proteins and to assess the role of human hemostatic proteins in viral replication.

Materials and Methods

Materials and Methods: The following viruses were chosen for comparison: influenza B (B/Astrakhan/2/2017), coronaviruses (Hcov229E and SARS-Co), type 1 adenovirus (adenoid 71), measles (ICHINOSE-BA) and rubella (Therien). The primary structures of viral proteins and 41 human hemostatic proteins were obtained from open–access www.ncbi.nlm.nih. gov and www.nextprot.org databases, respectively. Sequence homology was determined by comparing 12-amino-acid segments. Those sequences identical in ≥ 8 positions were considered homologous.

Results

Results: The analysis shows that viral proteins contain segments which mimic a number of human hemostatic proteins. Most of these segments, except those of adenovirus proteins, are homologous with coagulation factors. The increase in viral virulence, as in case of SARS-Co, correlates with an increased number of segments homologous with hemostatic proteins.

Conclusion

Conclusion: Hemostasis plays an important role in viral replication and pathogenesis. The conclusion is consistent with the literature data about the relationship of hemostasis and inflammatory response to viral infections.

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The authors declare that there are no conflicts of interest present.