jofinЖурнал инфектологииJournal Infectology2072-6732IPO “АIDSSPbR"10.22625/2072-6732-2013-5-1-69-74jofin-156Research ArticleОригинальные исследованияOriginal ResearchЦитофлуориметрический анализ содержания цитотоксических клеток у пациентов с хроническим гепатитом СFlow cytometry analysis of cytotoxic cells in patients with chronic hepatitis CСельковаМ. С.SelkovaM. S.

заочный аспирант Научно-исследовательского института гриппа, тел.: 8-921-323-69-24

selkovams@mail.ruНаучно-исследовательский институт гриппа, Санкт-ПетербургРоссияScience Research Institute of Influenza, Saint-PetersburgRussian Federation201315092014516974Copyright © Селькова М.С., 20142014Селькова М.С.Selkova M.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.niidi.ru/jofin/article/view/156

У пациентов с хроническим гепатитом С проведено исследование содержания различных популяций цитотоксических лимфоцитов методом проточной цитометрии. Выявлено значительное снижение функционального потенциала NKNK-клеток при определении экспрессии CD 107а на фоне нормального содержания NK - и NK T-клеток независимо от молекулярно-биологических характеристик вирусной инфекции.

In patients with chronic hepatitis C study the content of different populations of cytotoxic lymphocytes by flow cytometry. There was a significant decrease in functional capacity of NK -cells in determining the expression of CD 107 a picture of the regular numerical index NK -and NK T-cells independently of molecular-biological characteristics of viral infection.

хронический вирусный гепатит СNK -клеткиNK T-клеткиактивированные NK -клеткиchronic hepatitis CNK -cellsNK T-cellsactivated NK -cellsReferencesСелькова, М.С. Особенности содержания NK -клеток у больных хроническим гепатитом С / М.С. Селькова [и др.] // Медицинская иммунология. – 2012. – Т. 14, № 4–5. – С. 439–444.Селькова, М.С. Особенности содержания NK -клеток у больных хроническим гепатитом С / М.С. Селькова [и др.] // Медицинская иммунология. – 2012. – Т. 14, № 4–5. – С. 439–444.Ahlenstiel, G. Natural Killer Cells are Polarized towards Cytotoxicity in ChronicHepatitis C in an Interferon-α-Dependent Manner / G. Ahlenstiel [et al.] // Gastroenterology. – 2010. – V. 138 (1). – Р. 325–335.Ahlenstiel, G. Natural Killer Cells are Polarized towards Cytotoxicity in ChronicHepatitis C in an Interferon-α-Dependent Manner / G. Ahlenstiel [et al.] // Gastroenterology. – 2010. – V. 138 (1). – Р. 325–335.Alter, G. CD107a as a functional marker for the identification of natural killer cell activity / G. Alter, J.M. Malenfant, M. Altfeld // J. Immunol. Methods. – 2004. – V. 294 (1–2). – Р. 15–22.Alter, G. CD107a as a functional marker for the identification of natural killer cell activity / G. Alter, J.M. Malenfant, M. Altfeld // J. Immunol. Methods. – 2004. – V. 294 (1–2). – Р. 15–22.Amadei, B. Activation of natural killer cells during acute infection with hepatitis C virus / B. Amadei [et al.] // Gastroenterology. – 2010. – V. 138 (4). – P. 1536–1545.Amadei, B. Activation of natural killer cells during acute infection with hepatitis C virus / B. Amadei [et al.] // Gastroenterology. – 2010. – V. 138 (4). – P. 1536–1545.Bonorino, P. Fine characterization of intrahepatic NK cells expressing natural killer receptors in chronic hepatitis B and C / P. Bonorino [et al.] // J. Hepatol. – 2009. – V. 51, № 3. – P. 458–467.Bonorino, P. Fine characterization of intrahepatic NK cells expressing natural killer receptors in chronic hepatitis B and C / P. Bonorino [et al.] // J. Hepatol. – 2009. – V. 51, № 3. – P. 458–467.Dessouki, О. Chronic hepatitis C viral infection reduces NK cell frequency and suppresses cytokine secretion: Reversion by anti-viral treatment / О. Dessouki [et al.] // Biochemical and Biophysical Research Communications – 2010. – V. 393, Issue 2, №5. – P. 331–337Dessouki, О. Chronic hepatitis C viral infection reduces NK cell frequency and suppresses cytokine secretion: Reversion by anti-viral treatment / О. Dessouki [et al.] // Biochemical and Biophysical Research Communications – 2010. – V. 393, Issue 2, №5. – P. 331–337Gao, B. Liver natural killer and natural killer T cells: immunobiology and emerging roles in liver diseases / B. Gao, S. Radaevа, O. Park // Journal of Leukocyte Biology. – 2009. – № 86. – Р. 513–528.Gao, B. Liver natural killer and natural killer T cells: immunobiology and emerging roles in liver diseases / B. Gao, S. Radaevа, O. Park // Journal of Leukocyte Biology. – 2009. – № 86. – Р. 513–528.Kenna, T. NK T cells from normal and tumor-bearing human livers are phenotypically and functionally distinct from murine NK T cells / T. Kenna [et al.] // J. Immunol. – 2003. – V. 171, № 4. – P. 177 5–177 9.Kenna, T. NK T cells from normal and tumor-bearing human livers are phenotypically and functionally distinct from murine NK T cells / T. Kenna [et al.] // J. Immunol. – 2003. – V. 171, № 4. – P. 177 5–177 9.Liu, T.Y. Distinct subsets of human invariant NK T cells differentially regulate T helper responses via dendritic cells / T.Y. Liu [et al.] // Eur. J. Immunol. – 2008. – V. 38, № 4. – P. 1012–1023.Liu, T.Y. Distinct subsets of human invariant NK T cells differentially regulate T helper responses via dendritic cells / T.Y. Liu [et al.] // Eur. J. Immunol. – 2008. – V. 38, № 4. – P. 1012–1023.Mercer, J.C. Natural killer T cells: rapid responders controlling immunity and disease / J.C. Mercer, M.J. Ragin, A. August // The International Journal of Biochemistry & Cell Biology. – 2005. – V. 37. – Р. 1337–1343.Mercer, J.C. Natural killer T cells: rapid responders controlling immunity and disease / J.C. Mercer, M.J. Ragin, A. August // The International Journal of Biochemistry & Cell Biology. – 2005. – V. 37. – Р. 1337–1343.Pavio, N. The hepatitis C virus persistence: how to evade the immune system? / N. Pavio, M.M.C. Lai // J. Biosci. – 2003. – V. 28, № 3. – Р. 287–304.Pavio, N. The hepatitis C virus persistence: how to evade the immune system? / N. Pavio, M.M.C. Lai // J. Biosci. – 2003. – V. 28, № 3. – Р. 287–304.

The authors declare that there are no conflicts of interest present.