jofinЖурнал инфектологииJournal Infectology2072-6732IPO “АIDSSPbR"10.22625/2072-6732-2022-14-3-55-60jofin-1398Research ArticleОригинальное исследованиеOriginal ResearchПроблемы ПЦР-диагностики COVID-19Рroblems of PCR diagnostics OF COVID-19ГригорьеваТ. Д.GrigorievaT. D.

Григорьева Тамара Дмитриевна – заведующая Городского консультативно-диагностического центра (вирусологического), кандидат медицинских наук.

Санкт-Петербург; тел.: (812)246-06-16, +7-904-554-50-12

Saint-Petersburg

tamara.doc@mail.ruБелопольскаяМ. А.BelopolskayaM. А.

Белопольская Мария Андреевна – кандидат медицинских наук, врач-инфекционист поликлиники КИБ им. С.П. Боткина, научный сотрудник лаборатории хронических вирусных инфекций Института экспериментальной медицины, кандидат медицинских наук.

Санкт-Петербург; тел.: 8(812)777-80-12; +7-921-303-56-67

Saint-Petersburg

belopolskaya.maria@yahoo.comКлиническая инфекционная больница им. С.П. БоткинаРоссияClinical Infectious Diseases Hospital named after S.P. BotkinRussian FederationКлиническая инфекционная больница им. С.П. Боткина; Институт экспериментальной медициныРоссияClinical Infectious Diseases Hospital named after S.P. Botkin; Institute of Experimental MedicineRussian Federation202222102022143спецвыпуск5560Copyright © Григорьева Т.Д., Белопольская М.А., 20222022Григорьева Т.Д., Белопольская М.А.Grigorieva T.D., Belopolskaya M.А.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.niidi.ru/jofin/article/view/1398

На сегодняшний день адекватная и своевременная оценка числа заболевших – основа эффективных мероприятий, направленных на предупреждение распространения новой коронавирусной инфекции COVID-19. «Золотым стандартом» подтверждения инфицирования SARS-CoV-2 на сегодняшний день остается полимеразная цепная реакция с обратной транскрипцией в режиме реального времени.

Цель

Цель: проанализировать опыт работы городского вирусологического центра Клинической инфекционной больницы им. С.П. Боткина по обследованию на наличие коронавируса SARS-CoV-2 методом полимеразной цепной реакции в период с 2020 по 2022 г.

Материалы и методы

Материалы и методы. Проведена систематизация ПЦР-исследований на COVID-19 за период 2020–2022 гг. Всего было обследовано 221 901 человек, положительные результаты получены у 55 372 (24,95%). Среди контингентов обследованных больных преобладали пациенты, проходившие стационарное лечение в клинической инфекционной больнице им. С.П. Боткина.

Результаты

Результаты. В данном исследовании проанализированы возможные причины появления ложноположительных и ложноотрицательных результатов ПЦР. Показана корреляция числа положительных результатов с динамикой выявления новых случаев COVID-19 в СанктПетербурге в период пандемии 2020–2022 гг. Установлено, что доля пациентов, обследованных за период госпитализации более 3 раз, остается значительной. Этот факт требует самого пристального внимания, учитывая высокую стоимость и трудоемкость ПЦРисследований.

To date, an adequate and timely assessment of the number of cases is the basis of effective measures aimed at preventing the spread of COVID-19 infection. Real-time reverse transcription polymerase chain reaction (RT-PCR) remains the gold standard for confirming COVID-19.

The purpose of the work

The purpose of the work: to analyze the experience of the city virological center of the S.P. Botkin Clinical Infectious Diseases Hospital (Botkin Hospital) for the examination for the presence of SARS-CoV-2 coronavirus by PCR in the period from 2020 to 2022.

Materials and methods

Materials and methods. The systematization of PCR studies on COVID-19 for the period 2020-2022 was carried out. A total of 221,901 people were examined, positive results were obtained in 55,372 (24.95%). Among the contingents of the examined patients, patients who underwent inpatient treatment at the Botkin Hospital.

Conclusions

Conclusions. This study analyzed the possible causes of false-positive and false-negative PCR results. The correlation of the number of positive results with the dynamics of detection of new cases of COVID-19 in St. Petersburg during the 2020-2022 pandemic is shown. It has been established that the proportion of patients examined more than 3 times during the period of hospitalization remains significant. This fact requires the closest attention, given the high cost and laboriousness of PCR studies.

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The authors declare that there are no conflicts of interest present.