jofinЖурнал инфектологииJournal Infectology2072-6732IPO “АIDSSPbR"10.22625/2072-6732-2022-14-4-77-87jofin-1382Research ArticleОригинальное исследованиеOriginal ResearchРоль полиморфизма генов некоторых молекул иммунного ответа в развитии острого вирус-индуцированного бронхиолитаThe role of gene polymorphism of some immune response molecules in the development of acute virus-induced bronchiolitisБочкареваЛ. С.BochkarevaL. S.

Бочкарева Лариса Сергеевна – ассистент кафедры детских инфекций; тел.: 8(3022) 32-00-85 (*114)

Чита

Chita

larisa.bochkareva.1992@mail.ruМиромановаН. А.MiromanovaN. A.

Мироманова Наталья Анатольевна – заведующая кафедрой детских инфекций, д.м.н., доцент; тел.: 8(3022)32-00-85(*114)

Чита

Chita

detinf-chita@mail.ruМиромановА. М.MiromanovA. M.

Мироманов Александр Михайлович – заведующий кафедрой травматологии и ортопедии, д.м.н., профессор; тел.: 8(3022)41-11-18

Чита

Chita

miromanov_a@mail.ruЧитинская государственная медицинская академияРоссияChita State Medical AcademyRussian Federation2022181020221447787Copyright © Бочкарева Л.С., Мироманова Н.А., Мироманов А.М., 20222022Бочкарева Л.С., Мироманова Н.А., Мироманов А.М.Bochkareva L.S., Miromanova N.A., Miromanov A.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.niidi.ru/jofin/article/view/1382Цель

Цель: исследовать генетический полиморфизм молекул иммунного ответа (TNFα-308G>A (rs1800629), IL4-589C>T (rs2243250), IL10-592C>A (rs1800872), IL10-819C>T (rs1800871), IL10-1082G>A (rs1800896), IL-17A-197G>A (rs2275913), IL-17F-161His>Arg (rs763780), TLR2-753 Arg>Gln (rs5743708), TLR-6-249 Ser>Pro (rs5743810) и оценить их прогностическое значение в развитии острого вирус-индуцированного бронхиолита.

Материалы и методы

Материалы и методы. В исследование включены дети первого года жизни, средний возраст которых составил 4,2±3,7 месяца. Основная группа – 106 пациентов с острым вирусным бронхиолитом средней и тяжелой степени тяжести, чаще ассоциированным с респираторно-синцитиальным вирусом (56,6%). Группа контроля – 100 здоровых детей аналогичного возраста, не имевших признаков острой респираторной инфекции на момент обследования и не получавших пассивную иммунопрофилактику респираторно-синцитиальной инфекции. Генотипирование проведено методом полимеразной цепной реакции. Анализ результатов включал соответствие закону Харди – Вайнберга, χ2-тест, относительный шанс и его 95% доверительный интервал. Для оценки распределения заявленных полиморфизмов генов и их аллелей использовали общую (χ2-тест, df=2) и мультипликативную (χ2-тест, df=1) модели наследования.

Результаты

Результаты. Выявлено, что риск развития острого вирусного бронхиолита повышен по сравнению со здоровой популяцией у носителей следующих генотипов: СС, СТ гена IL10-819C>T (rs1800871), GG, АА гена IL-17A-197G>A (rs2275913), HisHis гена IL-17F-161His>Arg (rs763780), SerSer, SerPro гена TLR-6-249Ser>Pro (rs5743810), GG гена TNF-α-308G>A (rs1800629). Генотип ТТ гена IL10-819C>T (rs1800871) ассоциирован с высоким риском развития бактериальных осложнений (пневмонии) при вирусном бронхиолите. Носители генотипов АА, СС гена IL10-592C>A (rs1800872) имеют повышенную вероятность тяжелого течения вирусного бронхиолита.

Заключение

Заключение. Генетический анализ полиморфизма генов IL10-592C>A (rs1800872), IL10-819C>T (rs1800871), IL-17A-197G>A (rs2275913), IL-17F-161His>Arg (rs763780), TLR-6-249Ser>Pro (rs5743810), TNF-α-308G>A (rs1800629) может использоваться в качестве персонифицированного критерия развития острого вирус-индуцированного бронхиолита у детей, определения тяжести его течения и вероятности формирования осложнений.

The aim of research

The aim of research: To investigate the genetic polymorphism of immune response molecules (TNFα-308G> A (rs1800629), IL4-589C>T (rs2243250), IL10-592C> A (rs1800872), IL10-819C> T (rs1800871), IL10-1082G>A (rs1800896), IL-17A-197G> A (rs2275913), IL- 17F-161His> Arg (rs763780), TLR-2-753Arg>Gln (rs5743708), TLR-6-249Ser>Pro (rs5743810) and assess their prognostic value in the development of acute virus-induced bronchiolitis.

Materials and methods

Materials and methods. The study included children of the first year of life, whose average age was 4.2 ± 3.7 months. The main group consisted of 106 patients with moderate and severe acute viral bronchiolitis, more often associated with respiratory syncytial virus (56.6%). The control group consisted of 100 healthy children of the same age who had no signs of acute respiratory infection at the time of examination and did not receive passive immunoprophylaxis of respiratory syncytial infection. Genotyping was performed using the polymerase chain reaction method. The analysis of the results included the compliance with the Hardy-Weinberg law, the χ 2 test, the relative chance, and its 95% confidence interval. To assess the distribution of the claimed gene polymorphisms and their alleles, we used the general (χ2 test, df =2) and multiplicative (χ2 test, df =1) inheritance models.

Results

Results. It was revealed that the risk of developing acute viral bronchiolitis is increased compared to the healthy population in carriers of the following genotypes: CC, ST gene IL10-819C> T (rs1800871), GG, AA gene IL-17A-197G> A (rs2275913), HisHis gene IL-17F-161His> Arg (rs763780), SerSer, SerPro gene TLR-6-249Ser> Pro (rs5743810), GG gene TNF-α-308G>A (rs1800629). The TT genotype of the IL10-819C>T (rs1800871) gene is associated with a high risk of developing bacterial complications (pneumonia) in viral bronchiolitis. Carriers of genotypes AA, CC of the IL10-592C> A (rs1800872) gene have an increased likelihood of a severe course of viral bronchiolitis.

Conclusion

Conclusion. Genetic analysis of gene polymorphism IL10-592C> A (rs1800872), IL10-819C> T (rs1800871), IL-17A-197G> A (rs2275913), IL-17F-161His> Arg (rs763780), TLR-6-249Ser> Pro (rs5743810), TNF-α-308 G>A (rs1800629) can be used as a personalized developmental criterion acute virus-induced bronchiolitis in children, determining the severity of its course and the likelihood of complications.

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The authors declare that there are no conflicts of interest present.